精品学习网论文频道一路陪伴考生编写大小论文，其中有开心也有失落。在此，小编又为朋友编辑了“高效液相色谱法测定罗布麻茶中槲皮素的含量”，希望朋友们可以用得着!

Abstract：Objective To determine the content of quercetin in the Luobuma Tea by HPLC. Method The mobile phase consisted of methanol-0.4% phosphoric acid (50∶50). Kromasil C18 column (250 mm×4.6 mm, 5 μm) was used. The wavelength for measurement was at 371 nm and the temperature was at 30 ℃. Result The calibration curve was linear in the range of 5.98～47.84 g/mL for quercetin. The regression equation was A=7 750.68C+1 196.33 (r=0.999 7). Conclusion This method can be used to determine the content of quercetin in Luobuma Tea accurately.

Key words：Luobuma Tea;quercetin;HPLC;content determination

罗布麻茶为罗布麻Apocynum venetum L.的叶经烘干粉碎制成的单方制剂,具有平肝安神、清热利水作用。临床用于肝阳眩晕、心悸失眠、浮肿尿少,高血压病,神经衰弱,肾炎浮肿。现已被《卫生部药品标准(中药成方制剂)》收载,该标准含量测定项下规定以芦丁为对照品,采用分光光度法测定。鉴于该含量测定方法操作比较繁琐、重现性差,因此选择芦丁水解苷元槲皮素作为罗布麻茶含量测定的控制成分。本试验报道高效液相色谱法测定罗布麻茶中槲皮素含量的方法学研究,结果表明,该方法简便、准确、重现性好,可以控制罗布麻的质量[1]。

1 仪器与试剂

岛津LC-10ADvp高效液相色谱仪,SPD-10Avp紫外检测器,HT-230A柱温箱;Kromasil C18色谱柱(250 mm×4.6 mm,5 μm);岛津UV-2401紫外分光光度计;Sartorius BP211D电子天平(准确到0.01 mg)。槲皮素对照品(批号100081-200406,购自中国药品生物制品检定所)。甲醇为色谱纯,水为重蒸水,其余试剂均为分析纯。罗布麻茶样品(山东省百草药业有限公司提供)。

2 方法与结果